Part:BBa_K176011
pCon 1.00(J23100)->RBS+GFP+T
pCon 1.00(J23100)+I13504
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
MEASUREMENT
We measured five constitutive promoter family member ( J23100, J23101, J23103, J23107, J23109, J23115, )strength by ligated each with a GFP gene ( I13504) , and then moniter the OD600 and GFP expression. K176011: J23100 + GFP ( I13504 ) K176012: J23101 + GFP ( I13504) K176013: K176009( J23107) + GFP ( I13504) K176014: K176008( J23115) + GFP ( I13504) K176015: J23109 + GFP ( I13504) K176016: J23103 + GFP ( I13504)
model
To see the details of the model, please click [http://2009.igem.org/Team:USTC/Modeling/Model-1 here]
data
J23100+GFP
COLONY NUMBER | T(min) | OD600 | FLU(arbitrary) | ST.FLU(ustc_st.1) | FLU/OD600(arbitrary) | ST.FLU/OD600(ustc_st.1) |
Ⅰ | 0 | 0.071 | 132.07 | 527.9210137 | 1860.140845 | 7435.507235 |
Ⅰ | 30 | 0.076 | 144.49 | 577.5672543 | 1901.184211 | 7599.569135 |
Ⅰ | 60 | 0.101 | 157.6633333 | 630.2247805 | 1561.023102 | 6239.849312 |
Ⅰ | 90 | 0.093 | 160.8866667 | 643.1093523 | 1729.964158 | 6915.154326 |
Ⅰ | 120 | 0.116 | 176.33 | 704.8407083 | 1520.086207 | 6076.213003 |
Ⅰ | 150 | 0.122666667 | 185.0633333 | 739.7503031 | 1508.668478 | 6030.573123 |
Ⅰ | 180 | 0.153 | 197.8966667 | 791.0487535 | 1293.442266 | 5170.253291 |
Ⅱ | 0 | 0.07 | 144.1 | 576.0083143 | 2058.571429 | 8228.690205 |
Ⅱ | 30 | 0.075333333 | 136.1066667 | 544.0567081 | 1806.725664 | 7221.991701 |
Ⅱ | 60 | 0.084333333 | 144.92 | 579.2860855 | 1718.418972 | 6869.004966 |
Ⅱ | 90 | 0.092 | 157.4133333 | 629.22546 | 1711.014493 | 6839.407174 |
Ⅱ | 120 | 0.098 | 164.73 | 658.4722389 | 1680.918367 | 6719.104478 |
Ⅱ | 150 | 0.117 | 179.78 | 718.6313307 | 1536.581197 | 6142.148126 |
Ⅱ | 180 | 0.135666667 | 194.6566667 | 778.0975603 | 1434.815725 | 5735.362853 |
For the details of the definition of ST.FLU and the unit ustc_st1, please click here.
plot
Protocol
1. Streak a plate of the strain which contain one of the parts listed in pSB1A3 .
2. Inoculate two 3ml cultures of supplemented M9 Medium and antibiotic( Ampicillin 0.1mg/ml) with single colony from the plate.
3. Cultures were grown in test tubes(BIO BASIC INC.12ml Polypropylene Round-bottom Culture Tubes With Graduations And Dual Cap Cat.No:TD444) for 16hrs at 37℃ with shaking at 200rpm.
4. Cultures were diluted 1:100 into 3ml fresh medium and grown for 3hrs.
5. Measure the fluorescence(SHIMDZU SPECTROFLUOROPHOTOMETER RF-5301PC, 250ul quartz cell path length 10mm,501 nm excitation,514 nm emission,1.5nm slit width) and absorbance (HITACHI UV-VIS spectrophotometer U-2810 ,200ul quartz cell,path length 10mm,600nm,1.5 nm slit width) every 30 minutes in the next 4hrs.
n/a | pCon 1.00(J23100)->RBS+GFP+T |